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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore may possibly add to tumorigenesis. This protein was observed to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial part in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are described. Two further splice variants were being described but comprehensive nucleotide sequence information will not be but available.
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Blocking peptides are peptides that bind specifically to your target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.
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This guide outlines further more feasible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide not bind towards the epitope within the goal protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic occasion. Cyclin E types a posh with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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